Prevalence of Enteric Bacteria with R-Plasmids in a Community of Kathmandu Valley

  • B. R. Tiwari Central Department of Micro-biology, Tribhuvan University, Kirtipur Kathmandu
  • R. B. Adhikari Central Department of Micro-biology, Tribhuvan University, Kirtipur Kathmandu

Abstract

Sixty eight of samples  water, chicken faces, children stool were collected from Macche NarayanVillage near Kathmandu. All together 53 different bacterial isolates were isolated. Thirty seven isolates belonged to E.coli and rest of them were the member of different Gram negative rods. The epidemiological studies of these antibiotic resistant isolates were carried out by antibiogram and plasmid profiling method. Among the total isolates 56.66% were resistance to one or more than one antibiotics.Among them 32% E.coli were resistance to one or more than one antibiotics. Plasmid analysis of the isolates showed that plasmid of 98 M.Da. was most common. Conjugation experiment proved that 8.10% of total isolates harbor the conjugative type of plasmid. It was also found that numerous isolates from water sources, chicken faeces and children stool samples showed identical antibiogram pattern and plasmid profile. Hence, this study also proved the epidemiological route of drug resistant bacteria, which can be either from the contaminated water storage pots or from chicken faeces to human.