In Vitro Screening and Evaluation of Antimicrobial Activities of Some Medicinal Plants of Nepal

  • N. R. Pokhrel Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
  • R P. Adhikari Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
  • M. P. Baral Royal Nepal Academy of Science and Technology, GPO Box 3323, Kathmandu

Abstract

Ethanol extracts of 20 different medicinal plants of Nepal were screened for their antimicrobial activities against seven bacteria. Extracts, which were found to be sensitive during screening process, were evaluated quantitatively. Zones of inhibition and minimum inhibitory concentration of the extracts were determined by agar well diffusion and broth dilution methods respectively. Gram positive bacteria were relatively more susceptible to the ethanol extract of medicinal plants. Of 20 medicinal plants. Bauhinia variegata. Berberis aristata, Bergenia ligulata, Psidium guajava and Tenninalia chebula showed relatively broad-spectrum antibacterial activity.