Phytochemical Analysis and Antimicrobial Activity of Different Extracts of Leaf of Inula cappa(Buch.-Ham. Ex D. Don) DC.
AbstractDifferent crude extracts (hexane, dichloromethane, ethyl acetate, methanol and 50% aqueous methanol) of Inula cappa of Nepalese origin were screened for the presence of different classes of phytochemicals and their antibacterial activities were tested against E. coli and S. aureus using agar diffusion method. Methanol and 50% aqueous methanol extracts showed the maximum antibacterial activity against both bacteria. Total phenolic, total flavonoid and total carbohydrate contents were estimated and free radical scavenging activity of selected extracts were determined. Total phenolic content was measured spectrophotometrically by using Folin-Ciocalteu reagent, total flavonoid content by using aluminum chloride and total carbohydrate content by using anthrone method. Gallic acid was used as the standard for the calibration of phenolics, catechin for flavonoids and glucose for carbohydrates. The total phenolic content in 50% aqueous methanol extract was found to be the highest (258.70±1.47 mg GAE/g extract), the total flavonoid content in methanol extract of was found to be the greatest (263.04±0.25 mg CE/g extract) and the total sugar content in 50% aqueous methanol extract was found to be the highest (177.43±0.23 mg GE/g). The free radical scavenging activity of the selected extracts was evaluated by DPPH method and the IC50 values were calculated. The IC50 values of methanol and 50% aqueous methanol extracts were found to be less than 50 µg/ml. GC-MS analysis of hexane extract showed the presence of 25 major phyto-constituents. Among them, terpenoid were the major constituents. The other constituents were phytosterols, steroidal hydrocarbons, tocopherol, ester of fatty acids, phthalate and hydrocarbons.
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