Aflatoxin Contamination in Livestock Feeds and Feed Ingredients of Nepal

  • B B Khadka Department of Livestock Services. Harihar Bhawan. Kathmandu
  • B K Sinha Department of Veterinaty Microbiology, Bihar Veterinary Collrgc, Patna - 800014
  • K. C.P. Singh Department of Veterinaty Microbiology, Bihar Veterinary Collrgc, Patna - 800014
  • L. N. Prasad Department of Veterinary Pathology. Bihar Veterina College. Patna - 800014
  • S. B . Verma Deparrmrnt of Veterinary Pathology. Bihar Veterina College, Patna - 800014


Atlatoxins are secondary metabolites produced by moulds Aspergillu flavus and A. parasiticus, which are ubiquitous and frequently contaminate most of the agricultural and livestock food products. These toxins are potent hepatotoxins and carcinogens, and mainly cause liver cancer. With limited information available on the level of atlatoxin contamination in Nepalese livestock feeds and feed ingredients, its risk to human health is not extensively investigated. In this study, atlatoxin contamination in various feed ingredients 1 processed livestock and poultry feeds collected from different parts of Nepal was measured. Altogether, 100 samples comprising of 25 livestock feeds, 50 poultry feeds and 25 feed ingredients were collected and processed for aflatoxin assay. The percentage of samples with aflatoxin contamination was 80%. 74% and 72% respectively for livestock feeds, poultry feeds and feed ingredients, giving an overall contamination of 75%. Aflatoxin B1 was detected in all aflatoxin positive samples either alone or in combination with aflntoxin B2. The concentration of aflatoxin in positive samples ranged from trace to 300 mg/kg in livestock feeds. trace to 500 mg/kg  in poultry feeds and trace to 300 mgkg of feed ingredients. There was non significant difference (P>0.05) in contamination of aflatoxins between various feeds / ingredients examined (c22df= 0.48). Such a high level of aflatoxin is of great concern to livestock, poultry and human health. The need for regularand frequent checking of these commodities before consumption is emphasized