Effect of Growth Regulators on in vitro Propagation of Citrus limon, Citrus sinbensis, Fortunella sp. and Poncirus trifoliata

  • R. Niroula Biotechnology Section, Department of Plant Resources, Thapathali, Kathmandu


Tissue culture is a useful method for Citrus crop improvement and propagation. Cotyledonary nodes of Citrus limon, C. sinensis, Fortunella sp. and Poncirus trifoliata were cultured on Murashige and Skoog medium (MSM) supplemented with different concentrations of benzylamminopurine (BAP) and napthalene acetic acid (NAA).The MSM was found satisfactory Plant growth regulators were required at different concentrations for shoot multiplication and rooting. Roots were produced into shoots when transferred on sand..