Performance of Pinus Roxburghii Inoculated with Pure Culture of Four Indigenous Ectomycorrhizal Fungi

  • G. S. Vaidya Royal Nepal Academy of Science and Technology (RONAST), Khumaltar; Lalitpur
  • K. Shrestha Natural History Museum Tribhuvan University, Swoyambhu, Kathmandu


Many ectomycorrhizal fungi were isolated from the spores and sporocarps collected from different  pine forests of Nepal. Among them, Astraeus hygrometricus, Boletus sp., Lycoperdon pyrijorme and Pisolithus arrijus were compared for their effectiveness under natural field conditions.Many of them were subjected for pure culture synthesis and selected from the pot culture experiment. Four fungi were inoculated in pine nursery (Pinus roxburghiz) for their performance at two sites, Godawari and Chalnakhel. Their performance were observed after six, nine and twelve months of transplantation. The results revealed that inoculated seedlings attained better shoot height and had better root length than their non mycorrhizal counterparts. More lateral and mycorrhizal roots, more shoot weight and more root weight than non inoculated ones. The inoculated transplants had hgher survival rate in the field as compared to those whch were not inoculated. Among the above fungi, Pisolithus arrijus performed better as regards to growth and survival of seedlings.