Effect of Rhizobium leguminosarum biovar phaseoli Inoculation Alone and in Combination with Organic Fertilizers on Bean (Phaseolus vulgaris L.)

  • K. D. Yami Royal Nepal Academy of Science and Technology, GPO Box 3323, Kathmandu
  • S. Shakya Central Department of Life Sciences Purvanchal University, Biratnagar


The ability of leguminous plants to fix nitrogen symbiotically with rhizobia should be exploited in order to meet the demand of protein rich pulses for a developing country like Nepal. Establishment of effectively nodulated legume cannot in many situations be left to chance but requires the introduction of effectively strains of rhizobia into the soil at sowing.A pot experiment was conducted using different treatments viz. inoculation with Rhizobium leguminosarum biovar phaseoli alone and in combination with compost, and verrmcompost. Plants that had been inoculated with the Rhizobium grew faster, taller and looked more vibrant and healthy than the plants without the bacterial inoculation as control at the time of flowering. Application of vermicompost and compost along with the Rhizobium resulted in less number of root nodules indicating supression of nitrogen fixation in presence of high nitrogen content of the organic manures. The observation clearly showed that although nitrogen fixation was supressed in the presence of organic manure at the time of flowering of plant growth combination of Rhizobium and organic fertilizers enhanced ultimate yield and plant nitrogen at the time of harvest.